Process for preparing acyl-CoA synthetase LCF-18

Details Process for preparing acyl-CoA synthetase LCF-18 Process for preparing acyl-CoA synthetase LCF-18

1979-04-09

1980-10-21

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435 15, 435911, C12N 910

Patent

active

042295386

ABSTRACT:
Acyl-CoA synthetase, having a high activity to C.sub.16 -C.sub.18 long chain fatty acids, is obtained by culturing strains belonging to various genera. As is known, acyl-CoA synthetase, having a strong activity to C.sub.16 -C.sub.18 long chain fatty acids, is generally obtained from liver of rat; however, it has now been discovered that acyl-CoA synthetase can be obtained from microorganisms and this enzyme is called acyl-CoA synthetase LCF-18. By use of acyl-CoA synthetase LCF-18 of the present invention which has a high activity to C.sub.16 -C.sub.18 long chain fatty acids, serum non-esterified fatty acid of human beings can be accurately determined, and it is very useful for diagnosis of diabetes and so forth.

REFERENCES:
patent: 4071413 (1978-01-01), Takahashi et al.
Estroumza et al., Chemical Abstracts vol. 72, 96873x (1970).
Ray et al., Chemical Abstracts vol. 86, 52028k (1977).
Kamiryo et al., Chemical Abstracts vol. 88, 34332s (1978).

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