Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-08-26
1996-04-02
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 912, 435 9121, 435 913, 435 9132, 435 915, 4351723, 435948, 436501, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 935 17, 935 31, 935 78, 935 88, C12Q 168, C12P 1934
Patent
active
055039790
ABSTRACT:
The present invention provides a replicatable and hybridizable recombinant single-stranded RNA probe molecule comprising: a recognition sequence for the binding of an RNA-directed RNA polymerase; a sequence required for the initiation of product strand synthesis by the polymerase; and a heterologus RNA sequence inserted at a specific site in the internal region of the recombinant molecule and complementary to an oligo or polynucleotide of interest. This invention also provides methods for determining the presence of concentration of an oligo- or polynucleotide of interest in a sample and for simultaneously determining the presence or concentration of several different oligo- or polynucleotides of interest in a sample.
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Kramer Fred R.
Lizardi Paul M.
Marschel Ardin H.
The Trustees of Columbia University in the City of New York
White John P.
Zitomer Stephanie W.
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