Methods for improving the sensitivity of hybridization assays

Details Methods for improving the sensitivity of hybridization assays Methods for improving the sensitivity of hybridization assays

1996-02-07

1997-12-30

Myers, Carla J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536254, 935 78, C12Q 168

Patent

active

057028966

ABSTRACT:
Methods for improving the sensitivity of hybridization assays which reduce non-specific binding (NSB) and non-specific hybridization (NSH) are disclosed. The methods include a washing method utilizing tetraalkylammonium salts at high temperatures, and release methods in which a probe-target complex is released from a solid support and recaptured. Use of both the washing and release methods results in substantial reduction in NSB and NSH without performing several rounds of release and recapture of the target nucleic acids.

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