Surface immobilization of magnetically collected materials

Details Surface immobilization of magnetically collected materials Surface immobilization of magnetically collected materials

1995-08-18

1997-08-26

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 912, 435 71, 435 725, 435 79, 435261, 436526, 436501, 436518, 436807, 436824, 209214, 209217, 536 243, 5303881, C12Q 168, C12Q 170, G01N 3353

Patent

active

056609906

ABSTRACT:
A method for determining the presence and/or concentration of a target substance e.g. protein, nucleic acid, bioparticle etc. in a fluid sample is provided. The method disclosed combines elements of immunoassays, coated cup assays and magnetic particle separation to effect the quantitation and recovery of an analyte in solution. Also the method ensures the non-reorientation of magnetically collected material by linking the magnetic particles to a collection surface via a specific binding pair. This linkage immobilizes the magnetic-analyte-containing material and thus allows for vigorous washing and reagent addition without significant redistribution or displacement. Thus the assay of this invention offers the speed of diffusion controlled kinetics as in a ferrofluid assay, the speed of collection of labeled target substance as in a magnetic assay as well as the ability to magnetically monolayer the ferrofluid, all of which is combined with the ease of washing and signal detection found in a coated cup assay.

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