Chemistry: electrical and wave energy – Apparatus – Coating – forming or etching by sputtering
Patent
1978-03-24
1979-09-25
Prescott, Arthur C.
Chemistry: electrical and wave energy
Apparatus
Coating, forming or etching by sputtering
204180G, G01N 2728, G01N 3316
Patent
active
041690362
ABSTRACT:
A slab-gel loading system includes a prismatic chamber for filling a plurality of slab-gel holders simultaneously. Each slab-gel holder comprises a pair of spaced apart plates defining an intermediate volume for gel containment. The holders are vertically positioned in the chamber with their major surfaces parallel to the chamber end walls. A liquid inlet is provided at the corner between the bottom and a side wall of the chamber for distributing a polymerizable monomer solution or a coagulable colloidal solution into each of the holders. The chamber is rotatably supported so that filling can begin with the corner having the liquid inlet directed downwardly such that the solution is gently funneled upwardly, without mixing, along the diverging side and bottom surfaces. As filling proceeds, the chamber is gradually rotated to position the bottom wall in a horizontal mode. The liquid filling means includes a plastic envelope with a septum dividing it into two compartments for intermixing two solutions of different density and thereby providing a liquid flow having a density gradient. The resulting gels have a density gradient between opposite edges for subsequent use in electrophoresis separations.
REFERENCES:
patent: 3856655 (1974-12-01), Roberts
patent: 3932265 (1976-01-01), Hoefer
patent: 3989612 (1976-11-01), Kragt et al.
patent: 4035377 (1977-07-01), Detroy
patent: 4049534 (1977-09-01), Posner
Reid et al., "A Simple Apparatus for Vertical Flat-Sheet Polyacrylamide Gel Electrophoresis," Anal. Biochem., vol. 22, pp. 374-381, (1968).
O'Farrell, "High Resolution Two-Dimensional Electrophoresis of Proteins," Jrnl. of Biol. Chem., vol. 250, No. 10, pp. 4007-4021, (1975).
Anderson Norman G.
Anderson Norman L.
Carlson Dean E.
Glenn Hugh W.
Jackson Frank H.
Prescott Arthur C.
The United States of America as represented by the United States
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