Composition for hybridizing nucleic acids using single-stranded

Details Composition for hybridizing nucleic acids using single-stranded Composition for hybridizing nucleic acids using single-stranded

1993-06-16

1997-02-25

Elliott, George C.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435 912, 530358, C12N 912, C12P 1934, A61K 3816

Patent

active

056058241

ABSTRACT:
The present invention relates to an improved composition for hybridizing polynucleotides with complementary nucleic acid sequences. Specifically, it relates to a composition for of increasing the specificity of a polynucleotide hybridization reaction in the presence of single-stranded nucleic acid binding protein.

REFERENCES:
patent: 4889818 (1989-12-01), Gelfand et al.
Kurkel et al, Proc. Natl. Acad Sci. USA, v. 76, n. 12, Dec. 1979, pp. 6331-6335.
Muniyappa et al, Proc. Natl. Acad. Sci USA, v. 81, May 1984, pp. 2757-2761.
Scopes, Protein Purification, Springer-Verlag, 1982, pp. 40-42 and 194-197.

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