Bioconversions catalysed by the toluene monooxygenase of Pseudom

Details Bioconversions catalysed by the toluene monooxygenase of Pseudom Bioconversions catalysed by the toluene monooxygenase of Pseudom

1994-03-17

1997-02-25

Mosher, Mary E.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435 691, 530350, C07K 1421

Patent

active

056058233

ABSTRACT:
Disclosed and claimed are toluene monooxygenase (TMO) gene sequences from Pseudomonas mendocina KR-1, TMO proteins encoded by these sequences, recombinant plasmids containing such sequences, and microorganism host cells containing such plasmids. A five-gene and six-gene TMO gene cluster encode proteins that are useful in a variety of bioconversions. In particular, the TMO gene cluster is useful fort he preparation of p-hydroxyphenylacetic acid and indigo. In addition, the TMO gene cluster is useful for the degradative bioconversion of toxic compounds, such as trichloroethylene.

REFERENCES:
Whited, G. M. et al. 1991. Journal of Bacteriology, vol. 173, pp. 3010-3016.
Whited, G. M. et al. 1991. Journal of Bacteriology, vol. 173, pp. 3017-3020.
Whited Dissertatian Abstracts International vol. 47, No 5 p. 1861B, Nov. 1986.

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