DNA sequencing

Details DNA sequencing DNA sequencing

1990-08-21

1993-05-04

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, G01N 2726, B01D 5702

Patent

active

052078809

ABSTRACT:
To sequence DNA automatically, flourescently marked DNA are electrophoresed in a plurality of channels through a gel electrophoresis slab; wherein the DNA samples are resolved in accordance with the size of DNA fragments in the gel electrophoresis slab into fluorescently marked DNA bands. The separated samples are scanned photoelectrically with a laser and a sensor, wherein the laser scans with scanning light at a scanning light frequency within the absorbance spectrum of said fluorescently marked DNA samples and light is sensed at the emission frequency of the marked DNA. The light is modulated from said laser at a predetermined modulation frequency and fluorescent light emitted by said DNA bands at said modulation frequency is detected, whereby background noise from the medium through which the light is transmitted is discriminated against.

REFERENCES:
patent: 3533933 (1970-10-01), Strauch
Roberto Cotrufo, "High Sensitivity Method for Fluorofore Detection in Gradient Polyacrylamide Slab Gels through Excitation by Laser Light: Application to Glycoproteins Stained with Concanavalin A-Fluorescein Isothiocyanate", Analytical Biochemistry 134 (1983) 313-319.
J. E. Brayden & W. Halpern, "A High-Spatial-Resolution Laser Beam Densitometer for Quantitative Analysis of Polyacrylamide Gels", Analytical Biochemistry 130 (1983) 9-13.
John J. Kopchick et al., "Rapid Analysis of Small Nucleic Acid Samples by Gel Electrophoresis", Analytical Biochemistry 115 (1983) 419-423.
Piotr Naimski et al., "Quantitative Analysis of Different Conformational Forms of DNA Bound to the Dye, 4'6-Diomidine-2-phenylindole, and Separated by Gel Electrophoresis", Analytical Biochemistry 106 (1980) 471-475.
B. C. Hyman & T. W. James, "Detection of A & T-Rich DNA in Gels by Differential Fluorescence", Analytical Biochemistry 131 (1983) 205-210.
U. Moller et al., "Evaluation of Stained and Unstained Electropherograms by Photoacoustic Spectroscopy", Journal de Physique, Colleague C6 (1983) C6121-C6124.
U. Moller et al., "Evaluation of Electrophoregrams by Means of Photoacoustic Spectrosopy", Journal of Photoacoustics, 1(2) (1982) 309-316.

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