Method of setting up a flow cytometer

Details Method of setting up a flow cytometer Method of setting up a flow cytometer

1992-10-27

1994-05-24

Housel, James C.

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Particle count or volume standard or control

436 8, 436 63, 436800, 2524081, 2504612, 356 36, G01N 33547

Patent

active

053148240

ABSTRACT:
A method of setting up a flow cytometer using microbeads fluorescing at a plurality of wavelengths, control cells for each of said wavelengths, certified blank microbeads. The blank microbeads are run and the PMT voltages are adjusted so that the blank microbeads are on scale on the flow cytometer. The control cells are run and the photomultiplier tube voltages are adjusted so that the single population falls in selected channels. The fluorescent microbeads are run on the flow cytometer and the Target Channels for the flow cytometer are determined.

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