Chemistry of carbon compounds – Miscellaneous organic carbon compounds – C-metal
Patent
1975-08-27
1979-10-30
Schain, Howard E.
Chemistry of carbon compounds
Miscellaneous organic carbon compounds
C-metal
23230B, 260112B, 2601127, 2601237, 424 12, 424 85, 424 88, 427250, 428336, 428434, 428469, 428474, 435 7, C07G 700, G01N 2106, G01N 3316
Patent
active
041728270
ABSTRACT:
In carrying out a purification and/or concentration step of antigens or antibodies, a substrate is immersed in a first aqueous medium containing a specifically reacting antigen to coat said substrate with a monomolecular layer of said specifically reacting antigen. The resulting coated substrate is then immersed in a second aqueous medium containing immunologically reactive antibody specific to the antigen in the first aqueous medium to complex said immunologically reactive antibody with said specifically reacting antigen. The resulting substrate is then immersed in a reagent capable of cleaving the immunological bond between said immunologically reactive antibody and said specifically reactive antigen and forming a solution of said immunologically reactive antibody in the immunological bond-cleaving solution and leaving said specifically reacting antigen coated on said substrate. The method can be reversed for preparing a purified concentration of an immunologically reactive antigen whereby a specifically reacting antibody is substituted for the antigen and the corresponding immunologically reactive antigen is substituted for the antibody.
REFERENCES:
patent: 2666355 (1954-01-01), Trurnit
patent: 3171783 (1965-02-01), Fisk
patent: 3236732 (1966-02-01), Arquilla
patent: 3313706 (1967-04-01), Lembke et al.
patent: 3492396 (1970-01-01), Dalton et al.
patent: 3639558 (1972-02-01), Csizmas
patent: 3639559 (1972-01-01), Tax
patent: 3646346 (1972-02-01), Catt
patent: 3658982 (1972-04-01), Reiss et al.
patent: 3697645 (1972-10-01), Meier et al.
J. Am. Chem. Soc. 59, p. 1406 (1937), Langmuir et al.
Fed. Proceedings, vol. 30, pp. 1494-1502 (1971), Vroman et al.
Helvetica Chemica Acta., 54 (Fasc. 4), pp. 1208-1217 (1971), Rothen et al.
Press release, G. E., Nov. 22, 1972.
General Electric Company
Schain Howard E.
LandOfFree
Method for concentration and purification of antigens and antibo does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for concentration and purification of antigens and antibo, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for concentration and purification of antigens and antibo will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-19626