Method for cultivation of hepatocytes

Details Method for cultivation of hepatocytes Method for cultivation of hepatocytes

1998-06-05

2000-10-24

Lankford, Jr., Leon B.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Primate cell, per se

435383, 435384, 435404, 435405, C12N 500

Patent

active

061366005

ABSTRACT:
The present invention provides a method for cultivation of hepatocytes efficiently by using a hepatocyte growth promoting factor derived from 3T3 cells, which comprises culturing hepatocytes isolated from the liver of a matured mammal in a medium containing pleiotrophin, fetal bovine serum, ascorbic acid or analogues thereof and nicotinamide or an analogue thereof, thereby forming primary colonies of the hepatocytes; and a method for subculture of the primary hepatocytes in the same medium.

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