High-affinity salicylic acid-binding protein and methods of use

Details High-affinity salicylic acid-binding protein and methods of use High-affinity salicylic acid-binding protein and methods of use

1997-10-23

2000-10-24

Housel, James C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 723, 435200, 536 241, G01N 3353

Patent

active

061365521

ABSTRACT:
A high-affinity salicylic acid-binding protein (SABP2) derivable from tobacco and Arabidopsis is disclosed. The tobacco protein has a molecular weight of approximately 25 kDa and reversibly binds SA with an apparent K.sub.d of approximately 90 nM and a B.sub.max of 10 fmol/mg protein. The SABP2 of the invention may be used to identify analogues of SA. Analogues so identified may be used in plants to augment disease-resistance response pathways or other SA-sensitive processes in which SA plays a role. Possible examples include flowering and alternative respiration. The SABP2 of the invention may also be used to identify and clone a gene or cDNA that encodes it, which then may be used to generate transgenic plants having altered SABP2 levels.

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