Method for determining sugar chain structure

Details Method for determining sugar chain structure Method for determining sugar chain structure

1994-08-11

1996-03-19

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 14, 435 18, 435 24, 435 791, 435810, 536 185, 536 221, 536 254, C12Q 100, C12Q 154, C07G 300, C07H 1900

Patent

active

055003427

ABSTRACT:
A more exact, reliable, and simple method for determining the structure of a sugar chain, a kit to be used for this method, and novel oligosaccharides useful for the method as standard sugar chains are provided. The method for determining the structure of an N-acetyllactosamine type of sugar chain is characterized by determining the site of linkage and the mode of linkage of sugar residues at the non-reducing terminal side of a .beta.-N-acetylglucosamine residue linked to an .alpha.-mannose residue in M3 core by means of detecting the presence of the .beta.-N-acetylglucosamine residue after enzymatic or chemical treatment of the sugar chain. The kit for determining the structure of an N-acetyllactosamine type of sugar chain is characterized by containing at least one of the oligosaccharides represented by the chemical formula 1. The oligosaccharide is represented by the chemical formula 4.

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