Method of multiplex ligase chain reaction

Details Method of multiplex ligase chain reaction Method of multiplex ligase chain reaction

1996-12-18

1999-02-09

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 935 76, 935 77, 935 78, C12Q 168, C12P 1934, C12N 1500

Patent

active

058692525

ABSTRACT:
The invention relates to multiplex ligase chain reaction (LCR). Two or more purative target sequences are selected. For each one, a set of four probes is used simultaneously to amplify the putative sequence if it is present in the sample. Preferably, all the amplicons are labeled with a common label/hapten and, for each different target, with a unique label/hapten. The invention also relates to an immunochromatographic strip device and method employing a diagonal array of capture spots.

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