Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1993-11-29
1997-09-23
Moody, Patricia R.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
4351723, 4352404, 4353201, 530372, 800205, C12N 1529, C12N 514, C12N 1582
Patent
active
056706359
ABSTRACT:
The present invention relates, in general to protein that is a seed storage protein having high nutritional value. In particular, the invention relates to the protein AmA1 and to a DNA sequence encoding same. The invention further relates to a recombinant molecule comprising the AmA1 encoding sequence and to a host cell transformed therewith. In addition, the invention relates to a method for producing transgenic plants with high nutritionally rich amino acids.
REFERENCES:
Segura-Nieto et al 1992 (Sep.) J Agric Food Chem 40:1553-1558.
Gorinstein et al 1991 J Agric Food Chem 39:851-854.
de la Rosa et al 1992 (Jun.) J Agric Food Chem 40:931-936.
Gorinstein et al 1991 J Agric Food Chem 39:848-850.
Gen Bank Locus AHSSPBNQ, Accession Z11577S51155, 30 Jun. 1993.
Raina et al 1993 Mol Gen Genet 237:400-406.
Pir 45, Entry S24263, 20 Feb. 1995.
Pir 45, Entry A47185, 21 Sep. 1993.
Larkins, Genetic Engineering of Plants: An Agricultural Perspective, Genetic Engineering of Seed Storage Proteins (Plenium, NY), pp. 93-118 (1983).
Hoffman et al, Plant Mol. Biol., vol. 11, "A Modified Storage Protein is Synthesized, Processed, and Degraded in the Seeds of Transgenic Plants," pp. 717-729 (1988).
Guerche et al, Mol. Gen. Genet., vol. 221, "Expression of the 2S albumin from Bertholletia excelsa in Brassica napus," pp. 306-314 (1990).
Davies et al, The Biochemistry of Plants, A Comprehensive Treatise, vol. 1, The Plant Cell, Chapter 11, Ribosomes (Academic Press, New York) (1980).
Marchuk, Nucleic Acid Research, vol. 19, No. 5, "Construction of T-Vectors, A Rapid and General System for Direct Cloning of Unmodified PCR Products," p. 1154 (1991).
Paredes-Lopez et al, Lebensm. Wiss. U-Technol., vol. 21, No. 1, Isolation of Amaranth Proteins, pp. 59-61 (1988).
Maniatis et al, Molecular Cloning, A Laboratory Manual, Enzymes Used in Molecular Cloning, Restriction Enzymes, Cold Spring Harbor Laboratory, NY, pp. 104-106 (1982).
Ammerer, Methods in Enzymology, vol. 101, Recombinant DNA, Part C, "Expression of Genes in Yeast Using the ADCI Promoter," pp. 192-201 (1983).
Takaiwa et al, Plant Mol. Biol., vol. 16, "Analysis of the 5' Flanking Region Responsible for the Endosperm-Specific Expression of a Rice Glutelin Chimeric Gene in Transgenic Tobacco," pp. 49-58 (1991).
Broach et al, Experimental Manipulation of Gene Expression, Chapter 5, "Vectors for High Level, Inducible Expression of Cloned Genes in Yeast," pp. 83-117 (1983).
DeGreve et al, J. Mol. Appl. Genet., vol. 1, No. 6, "Nucleotide Sequence and Transcript Map of the Agrobacterium tumefaciens Ti Plasmid-Encoded Octopine Synthase Gene," pp. 499-511 (1982).
Rosahl et al, Mol. Gen. Genet., vol. 203, "Isolation and Characterization of a Gene from Solanum tuberosum Encoding Patatin, the Major Storage Protein of Potato Tubers," pp. 214-220 (1986).
Smith et al, Science, vol. 229, "Heterologous Protein Secretion from Yeast," pp. 1219-1229 (1985).
Horsch et al, Science, vol. 227, "A Simple and General Method for Transferring Genes into Plants," pp. 1229-1231 (1985).
Prestidge et al, Anal. Biochem., vol. 97, "Preparative Flatbed Electrofocusing in Granulated Gels with Natural pH Gradients Generated from Simple Buffers," pp. 95-102 (1979).
Laemmli, Nature, vol. 227, "Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4," pp. 680-685 (1970).
O'Farrell, J. Biol. Chem., vol. 250, No. 10, "High Resolution Two-Dimensional Electrophoresis of Proteins," pp. 4007-4021 (1975).
Elledge et al, Mol. Cell. Biol., vol. 7, No. 8, "Identification and Isolation of the Gene Encoding the Small Subunit of Ribonucleotide Reductase from Saccharomyces cerevisiae: DNA Damage-Inducible Gene Required for Mitotic Viability," pp. 2783-2793 (1987).
Towbin et al, Proc. Natl. Acad. Sci. USA, vol. 76, "Electrophoretic Transfer of Proteins from Polyacrylamide Gels to Nitrocellulose Sheets: Procedure and Some Applications," pp. 4350-4354 (1979).
Ausubol et al, Current Protocols in Molecular Biology, Phenol/SDS Method for Plant RNA Preparation (Greene Publishing Associates and Wiley-Interscience, New York, pp. 4.3.1-4.3.3 (1987).
Okayama et al, Methods in Enzymology, vol. 154, Construction and Screening of cDNA Libraries, "High-Efficiency Cloning of Full-Length cDNA; Construction and Screening of cDNA Expression Libraries for Mammalian Cells," pp. 3-28 (1987).
Anderson et al, Methods in Enzymology, vol. 96, "Immuno-precipitation of Proteins from Cell-Free Translations," pp. 111-120 (1983).
Ricciardi et al, Proc. Natl. Acad. Sci. USA, vol. 76, No. 10 "Purification and Mapping of Specific mRNAs by Hybridization-Selection and Cell-Free Translation," pp. 4927-4931 (1979).
Gubler et al, Gene, vol. 25, "A Simple and Very Efficient Method for Generating cDNA Libraries," pp. 263-269 (1993).
Young et al, Proc. Natl. Acad. Sci. USA, vol. 80, "Efficient Isolation of Genes by Using Antibody Probe," pp. 1194-1198 (1983).
Manfioletti et al, Nucleic Acids Res., vol. 16, No. 7, "A New and Fast Method for Preparing High Quality Lambda DNA Suitable for Sequencing," pp. 2873-2884 (1988).
Chamberlain, Anal. Biochem., vol. 98, "Fluorographic Detection of Radioactivity in Polyacrylamide Gels with the Water-Soluble Fluor, Sodium Salicylate," pp. 132-135 (1979).
Feinberg et al, Anal. Biochem., vol. 137, "A Technique for Radiolabeling DNA Restriction Endonuclease Fragments to High Specific Activity," pp. 266-267 (1984).
Rogers et al, Plant Mol. Biol. Manual D1, "Extraction of Total Cellular DNA from Plants, Algae and Fungi," eds. Gelvin et al, Kluwer Academic Publishers, pp. 1-8 (1994).
Higgins, Annu. Rev. Plant Physiol., vol. 35, "Synthesis and Regulation of Major Proteins in Seeds," pp. 191-221 (1984).
Perlman et al, J. Mol. Biol., vol. 115, "Localization of Genes for Variant Forms of Mitochondrial Proteins on Mitochondrial DNA of Saccharomyces cerevisiae," pp. 675-694 (1977).
Von Heijne, J. Mol. Biol., vol. 173, "How Signal Sequences Maintain Cleavage Specificity," pp. 243-251 (1984).
Raina et al, Proc. Natl. Acad. Sci. USA, vol. 89, "Molecular Cloning of a Gene Encoding a Seed-Specific Protein With Nutritionally Balanced Amino Acid Composition from Amaranthus," pp. 11774-11778 (1992).
Salinovich et al, Anal. Biochem., vol. 156, "Reversible Staining and Peptide Mapping of Proteins Transferred to Nitrocellulose after Separation by Sodium Dodecylsulfate-Polyacrylamide Gel Electrophoresis," pp. 341-347 (1986).
Biswas Subhra
Datta Asis
Raina Anjana
Clarke Dennis P.
Moody Patricia R.
LandOfFree
Seed storage protein with nutritionally balanced amino acid comp does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Seed storage protein with nutritionally balanced amino acid comp, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Seed storage protein with nutritionally balanced amino acid comp will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1939079