Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1983-08-26
1987-02-17
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 29, 435 39, 435177, C12Q 118, C12Q 102, C12Q 106, C12N 1102
Patent
active
046439684
ABSTRACT:
The metabolism and growth of cells is measured by suspending the cells in a liquid medium capable of forming a gel. The resultant suspension is formed into small droplets which droplets are caused to gel. Samples of the gel microdroplets (GMDs) are treated to alter the metabolism or growth of the cells, and the amount of metabolites, or other cellular products or external reaction products within each GMD is measured. The process can be utilized to determine essentially simultaneously the enumeration or count and the antibiotic susceptibility, or susceptibility to other compounds, factors, biological agents or other cells, of the cells which are representative of a larger sample of said cells.
REFERENCES:
patent: 4061466 (1977-12-01), Sjoholm et al.
patent: 4242447 (1980-12-01), Findl et al.
patent: 4391909 (1983-07-01), Lim
patent: 4401755 (1983-08-01), Weaver
Some Methods for Microbiological Assay, Board, R. G., and Lovelock, D. W., eds., Academic Press (1975), p. 23.
Cook Paul J.
Massachusetts Institute of Technology
Warden Robert J.
Wieder Stephen C.
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