Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1994-12-30
1997-09-23
Ziska, Suzanne E.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4352402, C12N 1500, C12N 500
Patent
active
056703511
ABSTRACT:
Methods, including culture media conditions, which provide for ex vivo human stem cell division and stable genetic transformation and/or the optimization of human hematopoietic progenitor cell cultures and/or increasing the metabolism or GM-CSF secretion or IL-6 secretion of human stromal cells are disclosed. The methods rely on culturing human stem cells and/or human hematopoietic progenitor cells and/or human stromal cells in a liquid culture medium which is replaced, preferably perfused, either continuously or periodically, at a rate of 1 ml of medium per ml of culture per about 24 to about 48 hour period, and removing metabolic products and replenishing depleted nutrients while maintaining the culture under physiologically acceptable conditions. Optionally growth factors are added to the culture medium.
REFERENCES:
patent: 5061620 (1991-10-01), Tsukamoto et al.
patent: 5399493 (1995-03-01), Emerson
Hoch et al. Nature 320: 275, 1986.
"Increased Gene Transfer Into Human Hematopoietic Progenitor Cells by Extended In Vitro Exposure to a Pseudotyped Retroviral Vector"; Christof von Kalle, et al; Blood, vol. 84, No. 9, Nov. 1, 1994, pp. 2890-2897.
Gail K. Naughton, et al.; Journal of Cellular Biochemistry; Hematopoeisis on Nylon Mesh Microenvironments; 19th Annual Meeting (1990).
Jerry Caldwell, et al.; Biotechnology Progress; Influence of Medium Exchange Schedules on Metabolic, Growth, and GM-CSF Secretion Rates of Genetically Engineered NIH-3T3 Cells; vol. 7, pp. 1-8; (1991).
Jerry Caldwell, et al.; Journal of Cellular Physiology; Culture Perfusion Schedules Influence the Metabolic Activity and Granulocyte-Macrophage Colony-Stimulating Factor Production Rates of Human Bone Marrow Stromal Cells; vol. 147, No. 2, pp. 344-353; (1991).
Richard M. Schwartz, et al.; Blood; In Vitro Myelopoiesis Stimulated by Rapid Medium Exchange and Supplementation with Hematopoietic Growth Factors; vol. 78, No. 12; pp. 3155-3161; (1991).
Richard M. Schwartz, et al.; Proceedings of the National Academy of Sciences; Rapid Medium Perfusion Rate Significantly Increases the Productivity and Longevity of Human Bone Marrow Cultures; vol. 88, No. 15; pp. 6760-6764; (1991).
Clarke Michael F.
Emerson Stephen G.
Palsson Bernhard O.
Schwartz Richard M.
The Regents of the University of Michigan
Ziska Suzanne E.
LandOfFree
Methods and compositions for the ex vivo replication of human he does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Methods and compositions for the ex vivo replication of human he, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods and compositions for the ex vivo replication of human he will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1938316