Electrochemical system with rotating electrode/sparger assembly

Chemistry: molecular biology and microbiology – Apparatus – Bioreactor

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204403, 204212, 204277, 435313, 435315, 435316, 435817, C12M 134

Patent

active

046056262

ABSTRACT:
An electrochemical system for determining the concentration of a bacteria or other enzymatic agents in a liquid sample (14). The system includes mounting means (20) for mounting an electrode assembly (16), and drive means for rotating the electrode assembly. The electrode assembly comprises a shaft (22) having a longitudinal axis and a longitudinally extending interior passage (40), an electrode (24) mounted at the lower end of the shaft, and sparging means (26) extending laterally from the lower end of the shaft. The sparging means is in communication with passage (40) through openings (50). The electrode and sparging means are mounted such that they are immersed in the sample when the shaft is mounted by the mounting means. The electrode assembly further comprises conductor means (44, 46) for electrically connecting the electrode to the electrochemical system. The electrode assembly is rotated during measurement of the concentration of the enzymatic agent. Prior to such measurement, dissolved oxygen may be rapidly removed from the sample by rotating the electrode assembly and introducing an inert gas into the passage, such that the inert gas passes from the rotating sparging means into the sample.

REFERENCES:
patent: 646313 (1900-03-01), Rhodin
patent: 1368362 (1921-02-01), Sill
patent: 3506544 (1970-04-01), Silverman et al.
patent: 4399028 (1983-08-01), Kile et al.
patent: 4434249 (1984-02-01), Ballestrasse et al.
Ser. No. 628,518, Silverman, filed 7/6/84.

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