Time-stable liquid cholesterol assay compositions

Details Time-stable liquid cholesterol assay compositions Time-stable liquid cholesterol assay compositions

1991-09-09

1993-06-08

Rosen, Sam

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving cholesterol

435 28, C12Q 160

Patent

active

052178730

ABSTRACT:
There is provided a stable cholesterol assay composition which comprises an aqueous solution of at least one bile acid or salt thereof being present in an amount of up to about 5 mM; a nonionic surfactant present in an amount of from about 0.15 to about 1.5 percent volume by volume; a buffer in a concentration of from 0 to about 65 mM; and cholesterol oxidase in a concentration of at least about 0.1 KIU/l. Solution pH is from about 5.5 to about 8.5. Addition of cholesterol esterase, phenol, peroxidase and 4-aminoantipyrine provides a total cholesterol chromogen system.

REFERENCES:
patent: 4116773 (1978-09-01), Polito

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