Method of producing follicle-stimulating hormone

Details Method of producing follicle-stimulating hormone Method of producing follicle-stimulating hormone

1972-12-07

1976-08-03

Friedman, Stanley J.

Drug, bio-affecting and body treating compositions

Fermentate of unknown chemical structure

Having a known elemental analysis

424109, A61K 3546

Patent

active

039730042

ABSTRACT:
A method of producing follicle-stimulating hormone (FHS) from sheep pituitary glands by disintegrating the pituitaries, extracting the latter with an approximately 0.2 molar solution of ammonium sulphate followed by bringing the pH of the solution to 5.5, separating the thusobtained extract into a supernatant and liquid and precipitate. Purification of FSH contained in the supernatant liquid is carried out by saturating the liquid with dry ammonium sulphate until a concentration of ammonium sulfate in the obtained solution of 40-50 percent of saturation is obtained the pH of the solution is adjusted to 7.4-7.5 and the resultant precipitate is separated from the supernatant liquid which is saturated with dry ammonium sulphate to a concentration in the obtained solution of 70-80 percent of saturation to obtain a precipitate which is treated with the ammonium sulphate solutions whose concentration is selected from the range of 56-58 percent of saturation, to form filtrates and subsequently precipitate FSH from the filtrates by saturating these with dry ammonium sulphate to a concentration thereof equal to 70-80 percent in the solutions obtained (of concentrated one), with subsequent bringing the pH of the solutions to 4.5 and isolation of the purified hormone from the supernatant liquid.

REFERENCES:
patent: 2256920 (1941-09-01), Allers et al.
patent: 2312901 (1943-08-01), Riehen et al.
patent: 2356803 (1944-08-01), Van Dyke et al.
Weisenberg et al., Biochemistry vol. 7, No. 12, pp. 4466-4479 Dec., 1968.

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