Method for detecting a nucleotide at a specific location within

Details Method for detecting a nucleotide at a specific location within Method for detecting a nucleotide at a specific location within

1991-12-03

1995-02-21

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 435808, 436501, 536 221, 536 231, 536 2431, 536 2432, 536 2433, 935 78, 935 88, C12Q 168

Patent

active

053914805

ABSTRACT:
A novel technique for determining the existence or nonexistence of a test nucleotide on a strand of DNA is provided. The determination advantageously uses an exonucleolytic agent that is capable of retaining a labeled nucleotide in a primer if there is a match between the test nucleotide on the strand of DNA and the complementary nucleotide on the primer, but not if there is a mismatch. The presence or absence of the test nucleotide then may be established by determining whether the label is preserved or lost following the reaction.

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Saiki et al. (1988) The New England J. of Med., vol. 319, No. 9, pp. 537-541.
Saiki et al. (1988) Abstract 0377 of the Annual Meeting of the Am. Soc. of Hum. Genet., 13.2.
Matthews et al. (1988) Anal. Biochem. vol. 169, pp. 1-25.

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