Preparation of protein membranes containing microbial cells

Details Preparation of protein membranes containing microbial cells Preparation of protein membranes containing microbial cells

1973-02-26

1976-08-03

Naff, David M.

Textiles: fiber preparation

195 31R, 195 54, 195 57, 195 59, 195 63, 195 68, 195DIG11, C07G 702, C12K 100

Patent

active

039727769

ABSTRACT:
Enzymatically active Protein-whole microbial cell complex membranes suitable for effecting enzymatic reactions are prepared by forming a dispersion containing synthetic polypeptide or natural protein macromolecules and whole microbial cells, casting a membrane from the dispersion and drying the membrane. In an alternative embodiment, the membranes are formed by electro-codeposition from a dispersion containing the macromolecules and cells. It is desirable to pre-treat the microbial cells to destroy proteolytic activity of the cells to prevent the cells from autolyzing and from destroying the membrane.

REFERENCES:
patent: 2920000 (1960-01-01), Hochstadt et al.
patent: 3705084 (1972-12-01), Reynolds
patent: 3709808 (1973-01-01), Hammond, Jr.
patent: 3758396 (1973-09-01), Vieth et al.
Brown, A.D., The Development of Halophilic Properties in Bacterial Membranes by Acylation Bioch. Mica. et., Biophysica Acts., vol. 93, 1964, pp. 136-142.
Hough, et al., Couplings of Enzymes onto Microorganisms, Nature vol. 235, 1972, p. 389.

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