Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Hormones – e.g. – prolactin – thymosin – growth factors – etc.
Patent
1986-08-25
1988-03-15
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Hormones, e.g., prolactin, thymosin, growth factors, etc.
435253, 435849, 530397, 530403, 530420, 530344, C07K 302, A61K 3724, A61K 3736
Patent
active
047314400
ABSTRACT:
A method for solubilization and naturation of somatotropin protein from refractile bodies of host cells is disclosed. The method embraces the discovery that an aqueous solution of urea, or dimethylsulfone, or mixtures of urea and dimethylsulfone, can be effectively used to solubilize refractile bodies containing such somatotropin protein. Once solubilized, somatotropin protein can be natured in an aqueous solution of urea, or dimethylsulfone, or mixtures of urea and dimethylsulfone, by contacting the solution with a mild oxidizing agent for a time sufficient to result in the formation of the disulfide bonds. Naturation can efficiently occur even at high protein concentration, in an impure preparation and in the absence of reducing agent.
REFERENCES:
patent: 4652630 (1987-03-01), Bentle et al.
Bentle Larry A.
Mitchell James W.
Shimamoto Grant T.
Storrs Stephen B.
Beck George R.
Beusen Jon H.
Cole Arnold H.
Monsanto Company
Schain Howard E.
LandOfFree
Method of somatotropin solubilization using dimethylsulfone and does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of somatotropin solubilization using dimethylsulfone and , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of somatotropin solubilization using dimethylsulfone and will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1927241