Simplified sequential chemiluminescent detection

Details Simplified sequential chemiluminescent detection Simplified sequential chemiluminescent detection

1998-04-22

2000-05-30

Ceperley, Mary E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 78, 435 79, 435 795, 435973, C12Q 168, G01N 3353, G01N 33535, G01N 33545, G01N 33552

Patent

active

06068979&

ABSTRACT:
A method for sequential chemiluminescent detection of two differently labeled analytes on a single blot is described. In the method, a uniquely labeled DNA is detected with a horseradish peroxidase (HRP) substrate followed by the detection of another uniquely labeled DNA with a second different enzyme substrate which also inhibits the chemiluminescence generated by HRP. The sequential detection method described herein eliminates the need to strip and reprobe Southern, Northern and Western blots. Potential applications of this method include forensic DNA fingerprinting where more than one probe is used for probing a Southern blot, multiplex DNA sequencing of more than one template, detection of gene rearrangements, mutations and gene linkage.

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L. Au, K. Chang, C. Shih, G. Teh, BioTech. 16(4) 680-683 (1994).

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