Promoters for use in aspergillus

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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435203, C12N 1556, C12N 1511

Patent

active

052527263

ABSTRACT:
A process for expression of a protein product in Aspergillus is disclosed. The process comprises transforming an Aspergillus strain with a vector system comprising DNA-sequences encoding a promoter including upstream activating sequences derived from an A. niger amylase, a suitable marker for selection of transformants, and a DNA-sequence encoding the desired protein product. The process enables industrial production of many different polypeptides and proteins in Aspergillus, preferably A. niger. Examples of such products are chymosin or prochymosin and other rennets, proteases, lipases and amylases. Also disclosed is an effective promoter for expression of a protein in Aspergillus, preferably Aspergillus niger being derived from a gene encoding an A. niger amylase. The A. niger amylases are the neutral and acid stable .alpha.-amylases and a new amylase not so far described and designated XA amylase. Also disclosed is the novel amylase from A. niger XA amylase.

REFERENCES:
Nakajima et al. (1986), Appl. Microbiol. Biotechnol., vol. 23, pp. 355-360.

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