Immunoassay for physostigmine

Details Immunoassay for physostigmine Immunoassay for physostigmine

1992-03-19

1993-10-12

Wax, Robert A.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Separation of immune complex from unbound antigen or antibody

436543, 436545, 436547, 436804, 424 88, 5303898, 435 71, G01N 33531, G01N 33534, G01N 3353, A61K 3514

Patent

active

052524957

ABSTRACT:
A radioimmunoassay for physostigmine in biological fluids. The method disclosed can detect 100 pg of physostigmine per ml of biological fluid using 0.1 ml of biological fluid. The antibody used in this method can be raised from an antigen produced by either or two methods--diazotizing para-aminobenzoic acid and reacting the diazotized material with physostigmine to form a physostigmine-hapten solution, which is then reacted with bovine serum albumin or by using a Mannich reaction to directly conjugate physostigmine to bovine serum albumin. The antibody used in the disclosed method has a cross-reactivity with the major metabolites of physostigmine of less than 1%.

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