Method of treating collected mammal semen and separating sperm i

Classifying – separating – and assorting solids – Precedent preparation of items or materials to facilitate... – Sorting special items or sorting by methods and apparatus...

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209578, 209606, 23230B, B07G 502

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active

043622461

ABSTRACT:
A method of treating mammal semen for use in artificial insemination is provided. Human saliva is added to the semen as an anti-coagulant. For mammals other than primates and voles, sperm-free human semen is added to the mammal semen as a cell membrane diffusion material so that a dye such as quinicrine or quinicrine mustard will be absorbed by the Y chromosome. The semen is then fed in a narrow stream through a detection chamber where it is irradiated with ultraviolet light causing the Y chromosomes to fluoresce. The stream is fed from the detection chamber through a fluidic amplifier wherein the stream is switched in response to whether the detected chromosome is fluorescent or non-fluorescent so that the fluorescent Y chromosome is directed through one outlet port and collected and the non-fluorescent X chromosome is directed through a second outlet port and collected.

REFERENCES:
patent: 3791517 (1974-02-01), Friedman
patent: 3976197 (1976-08-01), Bhattacharya
patent: 4155831 (1979-05-01), Bhattacharya
patent: 4225405 (1980-09-01), Lawson

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