Selective production of L-serine derivative isomers

Details Selective production of L-serine derivative isomers Selective production of L-serine derivative isomers

1987-08-13

1992-04-07

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing alpha or beta amino acid or substituted amino acid...

C12P 1306

Patent

active

051027925

ABSTRACT:
L-Serine derivatives produced by the enzyme catalyzed aldol condensation of glycine and an aldehyde in aqueous solution are recovered in high yield by extracting the aqueous product solution containing said serine derivatives with an organic phase comprising (i) an aldehyde, or (ii) mixtures of an aldehyde and a water immiscible organic solvent, followed by re-extracting the organic phase with an aqueous phase having a pH of less than about 7.0. The L-erythro isomers of L-serine derivatives such as L-phenylserine may be preferentially prepared by the use of this extraction/re-extraction procedure in combination with bioreactor reaction conditions which include a pH of from about 7.5 to 10, an aldehyde concentration of from about 1 to about 90 grams/liter, a glycine concentration of from about 10 to about 300 grams/liter, and a molar ratio of glycine to aldehyde of from about 4:1 to about 100:1.

REFERENCES:
patent: 3871958 (1975-03-01), Nakazawa et al.
patent: 4172846 (1979-10-01), Boesten
patent: 4605759 (1986-08-01), Mita et al.
patent: 4710583 (1987-12-01), Chmurny et al.
Nucleic Acids Res. (Plamann et al.), vol. 11, pp. 2065-2075 (1983).
J. Bacteriology (Schirch et al.), vol. 163, No. 1, pp. 1-7 (1985).
Trends in Biotechnology (Hamilton et al.), vol. 3, No. 1, pp. 64-68 (1985).
Biochemistry (Ulevitch et al.), vol. 16, No. 24, pp. 5342-5350 and 5356-5369 (1977).
Biochemistry (Ching et al.), vol. 18, No. 5, pp. 821-829 (1979).

Affiliated with

Also associated with

Rating

Selective production of L-serine derivative isomers does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Selective production of L-serine derivative isomers, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Selective production of L-serine derivative isomers will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1894733