Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1988-04-25
1992-04-07
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 91, 536 26, 536 27, 536 28, 536 29, 436 94, 436501, 935 77, C12Q 168, G01N 3348, G01N 33566, C07H 1512
Patent
active
051027852
ABSTRACT:
The method described characterizes each DNA segment to be mapped by cleaving it to produce DNA fragments which are then end labeled with a reporter(s) specific to the end nucleotides of each fragment. The labeled fragments are again cleaved to produce short fragments which are separated according to size. The short fragments are analyzed as to report identify and size which is indicative of the character of each fragment. By derivatizing the cleaved ends of the primary cleaved fragments, the labeling may be delayed until the second cleavage. Prior to the labeling the derivatized fragments, all underivatized fragments are removed, the derivatized fragments being immobilized.
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Brenner Sydney
Livak Kenneth J.
E. I. Du Pont de Nemours and Company
Wax Robert A.
Zitomer Stephanie W.
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