Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1982-11-05
1985-11-12
Shapiro, Lionel M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
260121, 260122, 106205, C12N 936, C07G 700, C08L 500
Patent
active
045528454
ABSTRACT:
The present invention relates to a method for separating lysozyme from egg-white which has a lysozyme fraction and an albumin fraction, without destroying the commercial utility of the lysozyme fraction or the albumin fraction. The method comprises the step of preferentially, reversibly binding the lysozyme fraction of the egg-white to an affinity resin at about the natural pH of the egg-white by contacting the egg-white with the resin, separating the albumin fraction from the resin to leave the lysozyme fraction bound to the resin in a lysozyme-resin complex, and eluting the bound lysozyme from the resin to obtain a lysozyme solution and a reusable resin. The eluted lysozyme and the separated albumin fraction are further treated in separating processing lines to produce a commercial albumin fraction egg-white product and a commercial lysozyme product. The invention also deals with the preparation and compositions of suitable affinity resins for the reversible, preferential binding of lysozyme in egg-white.
REFERENCES:
Chibata et al., Journal of Chromatography, vol. 215, pp. 93-98, (1981).
Muzzarelli et al., Biotechnology and Bioengineering, vol. XX, pp. 87-94, (1978).
Cherkasov et al., Molekulyarnaya Biologia, vol. 1, No. 1, pp. 41-46, (1967).
Jensen et al., Eur. Journal of Biochemistry, vol. 26, pp. 305-312, (1972).
LandOfFree
Method for separating lysozyme from egg-white does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for separating lysozyme from egg-white, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for separating lysozyme from egg-white will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1880100