Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1995-04-07
1996-07-09
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352523, 43525233, 435232, C12N 121, C12N 504
Patent
active
055344321
ABSTRACT:
A method for controlling and modifying biopolymer synthesis by manipulation of the genetics and enzymology of synthesis of polyhydroxybutyrate (PHB) and polyhydroxyalkanoate (PHA) polyesters at the molecular level in procaryotic and eukaryotic cells, especially plants. Examples demonstrate the isolation, characterization, and expression of the genes involved in the production of PHB and PHA polymers. Genes encoding the enzymes in the PHB and PHA synthetic pathway (beta-ketothiolase, acetoacetyl-CoA reductase and PHB polymerass or PHA polymerase) from Zoogloea ramigera strain I-16-M, Alcaligenes eutrophus, Nocardia salmonicolur, and P. olevorans were identified or isolated and expressed in a non-PHB producing organism, E. coli. Specific modifications to the polymers include variation in the chain length of the polymers and incorporation of different monomers into the polymers to produce co-polymers with different physical properties.
Peoples Oliver P.
Sinskey Anthony J.
Massachusetts Institute of Technology
Patterson Jr. Charles L.
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