Method for purifying a physiologically active substance produced

Details Method for purifying a physiologically active substance produced Method for purifying a physiologically active substance produced

1985-11-19

1989-11-14

Teskin, Robin

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530416, 530415, 530350, 530351, 530828, 435 68, 435 70, C12P 2100, C12P 2102, C07K 1300

Patent

active

048809152

ABSTRACT:
An aqueous solution containing a physiologically active substance, which is produced by recombinant DNA technique and which has cytotoxic activity against L-M cells and is capable of inducing hemorrhagic necrosis of transplanted Meth A sarcoma in the BALB/c mouse, can be effectively, efficiently purified by column chromatography using a column packed with a dye-bonded crosslinked agarose gel.

REFERENCES:
patent: 4677063 (1987-06-01), Mark et al.
patent: 4677064 (1987-06-01), Mark et al.
patent: 4677197 (1987-06-01), Lin et al.
patent: 4770995 (1988-09-01), Rubin
Rubin et al., Proc. Natl. Acad. Sci., vol. 82, pp. 6637-6641, Oct. 1985 "Purification and Characterization of a Human Tumor Necrosis Factor from the Luk II Cell Line".
Fugnani et al., Biol. Abst., vol. 74, No. 68078, 1984, "Purification of Human Interleukin 2 to Apparent Homogeneity".
Ichimura et al., Biol. Abst., vol. 78, No. 93089, 1984, "Characterization of mouse natural killer cell activating factor . . .".
Pichyangkul et al., Biol Abst., vol. 80, No. 13337, 1985, "Cellular origin of human lymphotoxin and its purification".
Neame et al., Chem. Abst., vol. 97(21), No. 179, 9-12m, 1982, "Sepharose-immobilized triazine dyes as absorbents for human lymphoblastoid interferon purification".
"Methods in Enzymology", vol. 78, pp. 417-421, 431-447, 513-522 and 536-563 (1981).

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