Bone resorption assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 79, 435 792, 435 794, 435 7021, 43524027, 435975, 436518, 436540, 436548, 436808, 5303871, 5303881, 5303911, 5303913, 5303879, G01N 3353, G01N 33543

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057534500

ABSTRACT:
Compositions and methods are provided which are useful in determining the levels of the C-telopeptide of human Type I collagen. The levels of the C-telopeptide are used as a marker of collagen degradation in an individual, and thus serve as a sensitive and specific indicator of bone resorption. Assays for the C-telopeptide find a variety of uses, including use in diagnosing metabolic bone disorders such as osteoporosis or postmenopausal rapid bone losers, monitoring the efficacy of therapeutic regimens designed to treat such disorders, determining the extent of imbalances between bone formation and resorption.

REFERENCES:
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patent: 5532169 (1996-07-01), Eyre
patent: 5538853 (1996-07-01), Risteli et al.
Otter et al., "Type I Collagen .alpha.-1 Chain C-Telopeptide: Solution Structure Determined by 600-MHZ Proton NMR Spectroscopy and Implications for its Role in Collagen Fibrillogenesis", Biochemistry, vol. 27, pp. 3560-3567 (1988).
Ramirez et al., "Molecular Biology of the Human Fibrillar Collagen Genes", Ann. N.Y. Acad. Sci., vol. 543, pp. 109-116, (1987).
Robins, "An Enzyme-Linked Immunoassay for the Collagen Cross-Link Pyridinoline", Biochem J., 207:617-620 (1982).
Tromp et al., "Structure of a Full-Length cDNA Clone for the Prepro.alpha.1(I) Chain of Human Type I Procollagen", Biochem. J. 253:919-922 (1988).
Zuk et al., "Evaluation of an Immunoassay for Collagen Cross-links", J. Bone & Min. Res. 6:(Suppl. 1) 197 (1991).
Eyre et al., "Monitoring Bone Resorption in Early Post-Menopausal Women by an Immunoassay for Cross-Linked Collagen Peptides", J.Bone & Mineral Res. 6:(Suppl. 1) 201 (1991).
Hanson et al., "A Specific Immunoassay for Bone Resorption Based on Cross-Linked Collagen Peptides in Urine", J. Bone & Min. Res. 6:(Suppl. 1) 669 (1991).
Risteli et al., "Bone Resorption Assay Based on a Peptide Liberated During Type I Collagen Degradation", J. Bone & Min. Res. 6:(Suppl. 1) 670 (1991). Package Insert (Feb., 1992).

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