Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1990-09-21
2000-06-20
Prouty, Rebecca E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 691, 4353201, 435219, 435226, 435476, 435488, 536 232, 536 234, 530327, 530324, 530329, C12N 1562, C12N 1509
Patent
active
060776943
ABSTRACT:
The subject invention relates to a method of producing and purifying large quantities of a biosynthetic protein.
The gene which codes for the protease is placed between the binding domain of a gene which codes for a binding protein and a gene coding for the target protein of interest. The fused gene construct is inserted in an expression vector which is then introduced into a host cell.
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Louis, John M., Autoprocessing of the HIV-1 Protease Using Purified Wild-Type and Mutated Fusion Proteins Expressed at High Levels in Escherichia coli; Eur. J. Biochem., pp. 199-361-369, 1991.
Bedonelle et al. (1988) Eur. J. Biochem. vol. 171, pp 541-549.
Debouck et al. (1987) PNAS, vol. 84, pp 8903-8906.
S. Scholtissek et al., "A Plasmid Vector System for the Expression of a Triprotein Consisting of .beta.-Galactosidase, a Collagenase Recognition Site and a Foreign Gene Product", Gene 62(1): 55-64, Jan. 1988.
C.V. Maina et al., "An Escherichia coli Vector to Express and purify Foreign Proteins by Fusion to and Separation From Maltose-Binding Protein", Gene 74(2): 365-373, Dec. 1988.
D.B. Smith et al., "Single-Step Purification of Polypeptides Expressed in Escherichia coli as Fusions with Glutathiona S-Transferase", Gene 67(1): 31-40, Jul. 1988.
P.L. Darke et al., "Human Immunodeficiency Virus Protease: Bacterial Expression and Characterizationof the Purified Aspartic Protease", J. Biol. Chem. 264(4): 2307-2312, Feb. 1989.
Prouty Rebecca E.
The United States of America as represented by the Department of
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