Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1993-03-12
1995-06-20
Draper, Garnette D.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4352523, 4353201, 536 234, C12N 1562
Patent
active
054260361
ABSTRACT:
The tendamistat gene can be used for the construction of fused genes with which fusion proteins are expressed and excreted in Streptomycetes host cells. The tendamistat portion can be modified, in particular it can be C-terminal shortened. When a gene for a shortened proinsulin in which the insulin B chain is linked to the A chain only via Lys or Lys-Lys is coupled to the tendamistat gene, this gene construction is introduced into an expression vector, and the latter is used to transform a Streptomycetes host cell, there is expression and secretion of the corresponding fusion protein. The fusion protein can easily be cleaved to give insulin precursors because of correctly established disulfide bonds. Genetic structures which code for the signal sequence and about the first ten amino acids of tendamistat as well as a desired protein are expressed in streptomyces host cells with a high yield, and the fusion proteins are secreted into the medium.
REFERENCES:
patent: 4366246 (1982-12-01), Riggs
patent: 4411994 (1983-10-01), Gilbert et al.
patent: 4717666 (1988-01-01), Brawner et al.
patent: 4918007 (1990-04-01), Koller et al.
Koller et al., Bio/Technology 7:1055-1059 (1989).
Koller et al., J. Bacteriology 171(9):4953-4957 (1989).
Vertesy et al., Eur. J. Biochem. 141:505-512 (1984).
Suggs et al., PNAS USA 78(11):6613-6617 (1981).
Brawner, et al., Expression Systems, CC 036, UCLA Symp. Suppl. 14A p. 103 (1990).
Shang Chang, Methods in Enzymology 153:507-516 (1987).
Noack et al., Gene 68:53-62 (1988).
PNAS 83:6766-6770, Sep. 1986, This ex. of Secretion and processing of insulin precursors in yeast.
Koller Klaus-Peter
Riess Gunther
Uhlmann Eugen
Wallmeier Holger
Draper Garnette D.
Hoechst Aktiengesellschaft
Ulm John D.
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