Species-specific DNA probes for vibrio vulnificus methods and ki

Details Species-specific DNA probes for vibrio vulnificus methods and ki Species-specific DNA probes for vibrio vulnificus methods and ki

1992-05-28

1995-06-20

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 2432, C07H 2104, C12Q 168

Patent

active

054260256

ABSTRACT:
The present invention relates to species-specific DNA probes specific for Vibrio vulnificus and Vibrio cholerae. The DNA probes of the present invention specifically detects Vibrio vulnificus or Vibrio cholerae in a mixed bacterial sample based on unique ribosomal RNA nucleotide sequences. When the DNA probes of the present invention are tagged with a labeled molecule such as a fluorescent label, it affords direct and immediate visualization of individual bacterial cells, and a rapid method of detection of bacterial infection in humans and shellfish without culturing.

REFERENCES:
patent: 4582789 (1986-04-01), Sheldon, III et al.
Betzl et al., Applied Environ. Micro. 56(9), 2927-2929 (1990).
Amann, R. I. et al.: Flourescent-Oligonucleotide Probing of Whole Cells for Determinative, Phylogenetic and Environmental Studies in Microbiology, J. of Bacteriology, 172:762-770 (Feb. 1990).
Amann, R. et al.: Identification In Situ and Phylogeny of Uncultured Bacterial Endosymboints, Nature, 351:161-164 (May 9, 1991).
Tamplin, M. L. et al.: Enzyme Immunoassay for Identification of Vibrio vulnificus in Seawater, Sediment, and Oysters, Applied and Environmental Microbiology, 57(4):1235-1240 (Apr. 1991).
Ratner, M.: DNA Probes: The Marekt Versus the Magic, Bio/Technology, 6:1369-1370 (Dec. 1988).
DeLong, E. F. et al.: Flourescent, Ribosomal RNA Probes for Clinic Application: A Research Review, Research, 28:41-44 (May 1990).
Reeves, R. H. et al.: Rapid Detection of Vibrio vulnificus Using a Fluorescent rDNA Probe, 1992 ASM General Meeting, New Orleans, La., May, 1992 (Abstract).

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