Process for preparing 2-keto-L-gulonic acid

Details Process for preparing 2-keto-L-gulonic acid Process for preparing 2-keto-L-gulonic acid

1975-04-21

1976-12-21

Monacell, A. Louis

Textiles: fiber preparation

195111, C12D 106

Patent

active

039986977

ABSTRACT:
2-Keto-L-gulonic acid is prepared directly from D-glucose by microbial conversion utilizing mixed culturing on or mixed contacting with a medium containing D-glucose, employing at least two kind of microorganisms; 2,5-diketo-D-gluconic acid producing strains which belong to the genera of Acetobacter, Acetomonas and Gluconobacter and strains capable of converting the 2,5-diketo-D-gluconic acid into 2-keto-L-gulonic acid which belong to the general of Brevibacterium and Corynebacterium. Both the incubation of the microorganisms in a medium containing D-glucose and the direct contact of any products obtained from the cells of the microorganisms with the substrate may be used in the disclosed process. By-production of the undesired optical isomer, 2-keto-D-gluconic acid, of the intended product is effectively prevented by employing the mixed culturing or contacting because of the presence of the 2,5-diketo-D-gluconic acid producing strain or any products thereof in the medium during at least part of the entire process.

REFERENCES:
patent: 2948659 (1920-08-01), Shoemaker
patent: 3907639 (1975-09-01), Makover et al.
Sonoyama et al., "2-Keto-Gulonic Acid by Fermentation," Chemical Abstracts vol. 82, No. 15275k (1975).

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