Introduction of DNA into bacillus strains by conjugation

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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43525231, 4353201, 435440, C12P 2102, C12N 121, C12N 1564, C12N 1575

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active

060664738

ABSTRACT:
The present invention relates to methods of constructing recombinant Bacillus strains encoding a polypeptide of interest, in which method a DNA construct comprising a DNA sequence encoding the polypeptide of interest is introduced into a recipient Bacillus cell by conjugation from a donor cell.

REFERENCES:
patent: 5345818 (1994-09-01), Schafer et al.
patent: 5346818 (1994-09-01), Schafer et al.
patent: 5843720 (1998-12-01), Tangney et al.
Selinger et al. Mobilization of Closely Related Plasmids pUB110 and pBC16 by Bacillus Plasmid pX0503 Requires Trans-Acting Open Reading Frame .beta. J. Bacteriol. 172(6): 3290-3297, Jun. 1990.

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