Membrane affinity apparatus and purification methods related the

Details Membrane affinity apparatus and purification methods related the Membrane affinity apparatus and purification methods related the

1995-06-05

1997-11-04

Chin, Christopher L.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is organic

2101983, 21050021, 21050023, 21050041, 210638, 210656, 435 6, 435180, 435181, 435182, 4352871, 4352872, 4352881, 436161, 436178, 436518, 436531, 436532, 530413, 530417, G01N 33549, G01N 33543

Patent

active

056839162

ABSTRACT:
A method and apparatus for carrying out affinity purification of a ligate. The method comprising, (a) providing a ligate containing liquid to a first side of at least one porous hollow fiber membrane with a ligand immobilized thereto that binds and separates the ligate from the liquid, (b) withdrawing a first portion of the liquid from the first side of the porous hollow fiber membrane, (c) recirculating the first portion of liquid to the first side of the porous hollow fiber membrane, (d) repeating steps (a) to (c) until a majority of the liquid has flowed through the porous hollow fiber membrane, and (e) providing an elution solution to one side of the porous hollow fiber membrane under a pressure sufficient to cause the elution solution to flow into and through the membrane to effect disassociation of any ligate-ligand bonds wherein any ligate bound to the ligand is eluted with the elution solution.

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