DNA cloning vectors with in vivo excisable plasmids

Details DNA cloning vectors with in vivo excisable plasmids DNA cloning vectors with in vivo excisable plasmids

1989-04-20

1992-07-07

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4353201, C12N 1510, C12N 1570

Patent

active

051282569

ABSTRACT:
Vectors are described that circumvent traditional DNA cloning and subcloning procedures, and that contain a unique DNA cartridge that permits both cloning of DNA directly into DNA sequences present within the cartridge, and in vivo removal and circularization of the cartridge thereby yielding an autonomously replicating structure. Because the DNA cartridge can include a wide variety of functional DNA sequences, the cloned DNA can be subjected to a plethora of molecular biological procedures without having to remove the cloned DNA from the cartridge thereby obviating the need to perform additional subcloning techniques. A particularly useful example of this type of vector is bacteriophage lambda containing the DNA cartridge.

REFERENCES:
Poustka et al., Nature, vol. 325:353-357 (1987).
Brown et al. Journal of Biol. Chem. 258:8402-8412, 1983.
J. Abraham et al.; "DNA Sequence of Regulatory Region in Integration Gene of Bacteriophage .lambda."; P.N.A.S., vol. 77, May 1980; pp. 2477-2481.
E. Beck and B. Zink; "Nucleotide Sequence and Genome Organosation of Filamentous Bacteriophages Fl and Fd"; Gene, vol. 16, 1981; pp. 35-58.
S. Brenner et al.; "Plasmids: Hybrids Between ColEl Plasmids and E. Coli Bacteriophage Lambda"; Gene, vol. 17, 1982; pp. 27-44.
P. Dotto et al.; "The Functional Origin of Bacteriophage fl DNA Replication"; 1984; J. Mol. Biol., vol. 172, pp. 507-521.
F. S. Collins et al.; "Directional Cloning of DNA Fragments at a Large Distance from an Initial Probe: A Circularization Method"; P.N.A.S. vol. 81, Nov. 1984; pp. 6812-6616.
A. A. Melnikov, et al.; "Lamba Phagemids and Their Transducing Properties"; 1984; Gene vol. 28, pp. 29-35.
J. Karn et al., "Novel Bacteriophage .lambda. Cloning Vector"; P.N.A.S. vol. 77, Sep. 1980; pp. 5172-5176.

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