Assay method for phosphatidyl ethanolamine

Details Assay method for phosphatidyl ethanolamine Assay method for phosphatidyl ethanolamine

1988-03-31

1992-02-18

Nucker, Christine

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435189, 435191, C12N 906

Patent

active

050893939

ABSTRACT:
Ethanolamine in a sample can be assayed by treating the sample with ethanolamine oxidase, thereby to catalyze a reaction-consuming ethanolamine, oxygen and water, and forming glycolaldehyde, ammonia and hydrogen peroxide. The amount of consumed oxygen or the amount of generated ammonia or hydrogen peroxide is then determined, as a measure of the ethanolamine that was originally the sample. The ethanolamine can appear in the sample as such, or can be liberated simultaneously with or prior to the catalysis reaction, from an ethanolamine derivative, e.g. phosphatidyl ethanolamine by the action of phospholipase D. Ethanolamine oxidase can be produced from Bacillus sp. B-0783 FERM-P No. 5798 in a conventional culture medium, preferably by submerged aeration liquid culturation.

REFERENCES:
Taki et al., "Phospholipase D from Rat Brain" in Methods in Enzymology, Ed. by Lowenstein (N.Y. Acad Press, 1981), vol. 71, 746-50.
Narrod et al., "Metabolism of Ethanolamine", Journal of Bio. Chem., vol. 239, No. 7 (1964), pp. 2189-2193.

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