Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1992-12-18
1995-08-29
Chereskin, Che S.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
800DIG56, 43524045, 43524048, A01H 400
Patent
active
054459613
ABSTRACT:
This invention relates to the regeneration of fertile monocotyledonous plants from a plant composition that contains microspores. A method of the present invention comprises preculturing a plant composition under a plurality of stressful conditions that promote embryogenesis, isolating microspores at a temperature below about 25.degree. C., culturing the microspores in an embryoid/calli induction medium and regenerating a plant from an embryoid/calli. A solid, porous support system for transferring isolated microspores through a series of subcultures containing different media is also provided. The composition of the media are aspects of the invention.
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Genovesi Anthony D.
Yingling Richard A.
Chereskin Che S.
Dekalb Genetics Corporation
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