Method and compositions for the determination of serum calcium u

Details Method and compositions for the determination of serum calcium u Method and compositions for the determination of serum calcium u

1992-02-05

1993-06-01

Housel, James C.

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Blood serum or blood plasma standard or control

436 17, 436 74, 436 79, 436164, 436166, 2524081, 422 8209, G01N 3100, G01N 3320

Patent

active

052159221

ABSTRACT:
A colorimetric, particularly spectrophotometric, method and compositions for determining calcium levels in blood serum in the presence of magnesium and other serum ions is described. The method uses an aqueous composition with a pH between about 7.5 and 10 with arsenazo III to complex calcium and a hydroxyquinoline selected from the group consisting of 8-hydroxyquinoline and 8-hydroxyquinoline-5-sulfonate to complex magnesium. Lipase and a cyclodextrin can be used as reagents to clear triglycerides in the serum which can provide turbidity and false results in some instances. The method is particularly useful for human serum.

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