Nucleic acid amplification employing ligatable hairpin probe and

Details Nucleic acid amplification employing ligatable hairpin probe and Nucleic acid amplification employing ligatable hairpin probe and

1990-08-23

1993-06-01

Yarbrough, Amelia Burgess

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 91, 4351723, 4353201, 435805, 436501, 436508, 436538, 436815, 536 243, 536 2431, 536 2432, 935 2, 935 18, 935 19, 935 78, 935 88, 935110, C12P 1934, C12Q 168

Patent

active

052158993

ABSTRACT:
Specific nucleic acid sequences are amplified through the use of a hairpin probe which, upon hybridization with and ligation to, a target sequence is capable of being transcribed. The probe comprises a single stranded self-complementary sequence which, under hybridizing conditions, forms a hairpin structure having a functional promoter region, and further comprises a single stranded probe sequence extending from the 3' end of the hairpin sequence. Upon hybridization with a target sequence complementary to the probe sequence and ligation of the 3' end of the hybridized target sequence to the 5' end of the hairpin probe, the target sequence is rendered transcribable in the presence of a suitable RNA polymerase and appropriate ribonucleoside triphosphate (rNTPs). Amplification is accomplished by hybridizing the desired target nucleic acid sequence with the probe, ligating the target sequence to the probe, adding the RNA polymerase and rNTPs to the separated hybrids, and allowing transcription to proceed until a desired amount of RNA transcription product has accumulated. The amplification method is particularly useful in assays for the detection of particular nucleic acid sequences.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
Cantor et al. (1980) Biophysical Chemistry, Part III (W. H. Freeman & Co., U.S.A.) p. 1183.
Jendrossek et al. (1988) J. of Bect., vol. 170, No. 11 pp. 5248-5256.
Walker et al. (1984) Biochem J. vol. 224, pp. 799-815.
La Fanci et al. (1989) Bioch. and Biophysical Res. Comm. vol. 159, No. 1, pp. 297-304.
Sriprakesh et al. (1989) Gene Anal. Tech., vol. 6, pp. 29-32.
Krupp et al. (1987) Febs. Lett. vol. 242, No. 2, pp. 271-275.
Nucleic Acid Probes (CRC Press, U.S.A., 1989) Ed., Symons pp. 21, 22, and 30.
Szybelski (1985) Gene, vol. 40, pp. 169-173.

Affiliated with

Also associated with

Rating

Nucleic acid amplification employing ligatable hairpin probe and does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Nucleic acid amplification employing ligatable hairpin probe and, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Nucleic acid amplification employing ligatable hairpin probe and will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1813867