Reagent and method for determining total calcium in body fluids

Details Reagent and method for determining total calcium in body fluids Reagent and method for determining total calcium in body fluids

1974-08-09

1976-01-27

Reese, Robert M.

Chemistry: physical processes

Physical processes

Crystallization

252408, G01N 3122, G01N 3316

Patent

active

039349772

ABSTRACT:
A colorimetric determination of total calcium in serum and other body fluids comprising the steps of mixing the sample of body fluid with a liquid reagent consisting of 2,7-bis-(4 chloro-2-phosphonobenzeneazo)-1,8-dihydroxynapthalene-3,6-disulfonic acid, commonly known as chlorophosphonazo III, in a buffer system at pH 5.0 to 5.5, and thereafter spectrophotometrically measuring the level of color development. Potassium hydrogen phthalate is disclosed as a component of the buffer system.

REFERENCES:
ferguson et al., Anal. Chem. 36, 796, (1964).
Hackh's Chemical Dictionary, 4th Edn., McGraw-Hill, p. 538 relied on, 1969.

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