Fluorescent gram stain

Details Fluorescent gram stain Fluorescent gram stain

1984-10-01

1987-05-12

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 29, 435 36, 435802, 435808, 435837, 435849, 435852, 435857, 435882, 435883, 435885, 436800, 2504612, C12Q 104, C12Q 102, C12Q 114, G01N 2164

Patent

active

046650245

ABSTRACT:
A method to determine the Gram sign of microorganisms includes staining the microorganisms with a plurality of fluorescent dyes, applying excitation energy to the stained microorganisms, and observing the color of the fluorescence emission of the stained microorganisms. Gram-positive and Gram-negative microorganisms stain different colors, and assignment of the Gram sign may be made on the basis of the color of the stained microorganisms.

REFERENCES:
patent: 4094745 (1978-06-01), Scholefield
patent: 4126516 (1978-11-01), Messing et al.
patent: 4225669 (1980-09-01), Melnick et al.
patent: 4492752 (1985-01-01), Hoffman et al.
patent: 4508821 (1985-04-01), Mansour et al.
Matsuyama, T., Staining of Living Bacteria with Rhodamine 123, FEMS Micro. Letters, 21 (1984) 153-157.
Govorunov, et al., Microbiology (1982) 51, pp. 587-589, Study of Permeability of E. coli Membrane to Ethidium Bromide.

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