Immobilized Mucor miehe lipase for transesterification

Details Immobilized Mucor miehe lipase for transesterification Immobilized Mucor miehe lipase for transesterification

1987-07-31

1989-04-04

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

435180, 435198, 435931, C12P 764, C12N 1108, C12N 920, C12R 1785

Patent

active

048186953

ABSTRACT:
Immobilized lipase is produced by mixing an aqueous lipase solution with a particulate, macroporous, weak anion exchange resin, and recovering and drying the resin having lipase immobilized thereon. The resin has a particle size such that more than 90% resin particles have a size between 100-1000 .mu.m. The immobilized lipase is used in a packed bed for continuous transesterification or solvent free fats. Preferably, the lipase is Mucor miehei lipase.

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patent: 4170696 (1979-10-01), Hirohara et al.
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patent: 4472503 (1984-09-01), Matsuo et al.
"Preparation and Properties of Immobilized Lipases", J. Lavayre et al., Biotech & Bioeng, vol. XXIV, pp. 1007-1013 (1982), John Wiley & Sons, Inc.
"Ester Exchange of Triglyceride by Entrapped Lipase in Organic Solvent", K. Yokozeki et al., Poster, Enz. Eng. 6, Kashikojima, Japan, Sep. 20-25, 1981.
"Application of Immobilized Lipase to Regio-Specific Interesterification of Triglyceride in Organic Solvent", Euro. Journal Appl. Microbial Biotechnol (1982) 14:1-5.
"Application of Immobilized Lipase to Hydrolysis of Triacylglyceride" Y. Kimura et al., Euro. Journal Appl. Microbial Biotechnol. (1983) 17:107-112.

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