Method for producing oriented connective tissue cells

Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing

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4352841, 4352861, 4353031, 4352972, 73788, 73826, 606 57, C12M 300, G01N 300, A61B 1756

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active

057006881

ABSTRACT:
The present invention pertains to a mechanical testing apparatus for determining the mechanical properties of an oriented tissue-equivalent, while simultaneously providing a suitable cell culture environment. The mechanical testing apparatus comprises an incubator equipped with means for controlling the atmosphere and means for controlling the temperature inside said incubator. Located within the incubator chamber are means for securing, in a desired location, the oriented tissue-equivalent to be mechanically tested. Further, the apparatus is equipped with means for controlling the length of the oriented tissue-equivalent, means for determining the force generated by the oriented tissue-equivalent, means for determining the length of the oriented tissue-equivalent and means for conditioning and recording force and length measurements.

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patent: 5437668 (1995-08-01), Aronson et al.
patent: 5463902 (1995-11-01), Shrive et al.
Bell, E., et al., "Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro", PNAS USA, 76(3):1274-1278 (1979).
Weinberg, C.G. and Bell, E., "Regulation of Proliferation of Bovine Aortic Endothelial Cells, Smooth Muscle Cells, and Adventiial Fibroblasts in Collagen Lattices", J. Cell Physiol., 122:410-414 (1985).
Ehrlich, H.P., "Contraction of Collagen Lattice By Peritubular Cells From Rat Testis", J. Cell Sci., 82:281-294 (1986).
Weinberg, C.B. and Bell, E., "A Blood Vessel Model Constructed From Collagen and Cultured Vascular Cells", Science, 231:397-400 (1986).
Aggarwal, A., A Syngeneic Ligament Equivalent, Phase I: Mechanical . . . Lattice, B.S., Thesis M.I.T.

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