Chemistry: analytical and immunological testing – Biospecific ligand binding assay
Patent
1994-04-04
1998-02-17
Scheiner, Toni R.
Chemistry: analytical and immunological testing
Biospecific ligand binding assay
436518, 436536, 436537, 436538, 435973, 435967, G01N 3353
Patent
active
057190631
ABSTRACT:
Disclosed is a method of assaying and apparatus for assaying, in assay apparatus, for an analyte in a sample by specifically binding the analyte to a binding molecule having a detectable tag to form a complex, the presence of which can be determined quantitatively by measuring the amount of tag. In one embodiment, the sample is contacted in that apparatus with a complex of binding molecules so as to effect the specific binding. The complex has at least two differently tagged different binding molecules in a predetermined ratio, at least a first of which tagged binding molecules being that which will specifically bind to the analyte. The second tagged binding molecule is one which will not specifically bind to the analyte or to other moieties in the sample. The relative non-specific binding characteristics of those binding molecules with respect to said apparatus are in a known or predetermined relationship. Quantitative measurement of those tags coupled to bound quantities of the binding molecules is then made substantially simultaneously. From the known proportion of binding molecules in the test body, the known ratio between the non-specific binding characteristics of the binding molecules to the apparatus, and the quantitative measurement of the bound tags, one can relatively and accurately determine the specific binding of the first binding molecule to the analyte.
REFERENCES:
patent: 4582809 (1986-04-01), Block et al.
Boehringer Mannheim Corporation
Scheiner Toni R.
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