Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1993-03-08
1994-11-08
Fox, David T.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
43525411, 4353201, 435 691, 435 711, 536 232, 536 237, 536 241, 536 2374, C12N 1500, C12N 114, C07H 1700, C12P 2104
Patent
active
053626400
ABSTRACT:
This invention relates to a novel ornithine carbamoyl transferase (OCTase) gene, a recombinant DNA containing the OCTase DNA, a transformation system for a basidiomycete, and production of useful proteins using the system.
More particularly, this invention relates to the OCTase gene of Coriolus hirsutus to afford an efficient host-vector system in basidiomycetes (particularly a white rot fungus such as Coriolus hirsutus IFO 4917) for the preparation of useful proteins. OCTase is the enzyme to transform ornithine to citrulline in arginine biosynthesis in organisms. The present invention provides the OCTase genes of C. hirsutus, the useful Arg.sup.- auxotrophic mutant of C. hirsutus deficient in the OCTase gene, the efficient condition for the preparation of protoplasts and the transformation of the mutant with the cloned OCTase gene and the recombinant DNA's including a promoter, a signal peptide-coding DNA and a protein-coding DNA, the construction of the novel host-vector system of C. hirsutus, and the new method of a large scale preparation of useful proteins using this novel host-vector system with a new recombinant DNA technique.
Furthermore, this invention provides a highly efficient method to produce a useful protein such as lignin peroxidase which is difficult to produce by the conventional method.
REFERENCES:
Upshall et al. 1986. MGG 204:349-354.
Hahm et al. 1988. Appl. Environ. Microbiol. 54:1610-1611.
Kita Yukio
Matsufuji Mieko
Tsukamoto Akira
Fox David T.
Kemmerer Elizabeth C.
Oji Paper Co. Ltd.
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